The importance of SOC broth for bacterial transformation Article

The importance of SOC broth for bacterial transformation - Article Example The elementary one of it is Lysogeny Broth. SOC is associated with it (Hannan). SOC is a variant and a further refined form of SOB. While SOB is devoid of glucose, it is the presence of glucose to SOB which makes it a SOC. It also helps identifying and determining different colonies of bacteria, their growth time, and their overall nomenclature. SOC media is enriched with glucose which makes it more sought after for the formation and lateral transformation of bacteria. However the presence of bacteria is a concern since it strengthens the overall cell existence and ultimately more resistance. Other advantage to SOC broth is its adjustment to the PH level around 7, which is most desired and provides options towards a neutral usage. Apart from this, SOC contains certain percentage and proportion of MgSo4. Hence all these traits and possessions make SOC broth more suitable for bacterial transformation than the

Shakespeares Othello - Othello and Desdemona :: GCSE Coursework Shakespeare Othello

Othello and Desdemona In the play, The Tragedy of Othello, Shakespeare really tests our conception as to what love is, and where it can or can't exist. Judging from the relationship between Desdemona and Othello, the play seems to say that marriage based on an innocent romantic love or profane love is bound to fail. Shakespeare is pessimistic about the existence and survival of a true type of love. There is a common thread of betrayal and deceit among his female characters, especially. Othello and Desdemona, as portrayed in the play, are the two greatest innocents there ever were. The two appear to love one another romantically at first, but this romantic love becomes more of a profane love, or more likely was truly a profane love all along. This comes to pass because there is no foundation for a relationship here. There is no trust, no communication, and no understanding. Othello has spent most of his life in battle, which makes him good at some things-- namely, battle. Othello says "Rude am I in my spee ch,/ and little bless'd with the soft phrase of peace;/ for since these arms of mine had seven years' pith,/ Till now some nine moons wasted, they have us'd/ Their dearest action in the tented field;/ And little of this great world can I speak/ More than pertains to feats of broils and battle" (1113). Desdemona is little more that a girl, inexperienced in the ways of the world. She is taken in by Othello's war stories. Desdemona takes one look at the hunk of burning love that is Othello, his virility and manliness, and she is swept off her feet. But is this a true love? She speaks so fondly of him, yet hardly knows him. As she defends her newly born love for Othello, Desdemona says (among other things), "My downright violence, and storm of fortunes,/ May trumpet to the world. My heart's subdu'd/ Even to the very quality of my lord./ I saw Othello's visage in his mind,/ And to his honors and his valiant parts/ Did I my soul and fortune consecrate." (1118). I can say from experience t hat in the "Magic Time", the first part of the relationship, some things are said that maybe affected by Love's blindness. Put these two together, and you have the equivalent of a couple of kids playing doctor. The two big clumsy babies "fumbling towards ecstasy" might have actually made it if they were free from outside forces.

National Integration in India. Essay

National Integration is a feeling that binds together the citizens of our country and make us to think and act as if we are one. India is a land of diversity. There are many languages, religions, castes, races and cultures in India. Since ancient times, people belonging to different communities, races, religion and cultural groups have been living in harmony. Toleration is characteristic feature of India’s culture and heritage. During the Mughal and British rule, there had been many attempts to weaken this unity. Using the policy of divide and rule, they created a disunity among the people by instigating communal feelings. National Integration is of great significance in a country like India where varied cultures, religions and languages exist. Integration is not achieved in India because of communalism, castism and linguistic differences. The need for national integration is of utmost importance at this period of India’s history. National integration is very essential for social peace and harmony. No progress can be made in the field of art, literature, science, technology and economy without integration. India cannot play an effective role in the international arena unless she sets her own house in order. We cannot enjoy peace and security unless there is mutual trust and good will among all sections of our people. For national integration , it is very essential that people should give up communal and sectarial outlook. They should consider themselves as Indians first and Indians last .The evils of casteism and linguistic isolation should be rooted out. Economic inequalities among the people should also be removed. Only in this manner can we lay the foundation of a strong and united India.

Malcolm X, Black Nationalist and Civil Rights Activist

Malcolm X (May 19, 1925–February 21, 1965) was a prominent figure during the Civil Rights era. Offering an alternative view to the mainstream Civil Rights movement, Malcolm X advocated for both the establishment of a separate black community (rather than integration) and the use of violence in self-defense (rather than non-violence). His forceful, uncompromising belief in the evils of the white man frightened the white community. After Malcolm X left the black Muslim Nation of Islam organization, for which he had been both a spokesperson and a leader, his views toward white people softened, but his core message of black pride endured. After Malcolm X was assassinated in 1965, his autobiography continued to spread his thoughts and passion. Fast Facts: Malcolm X Known For: Major figure in the African American Civil Rights movementAlso Known As: el-Hajj Malik el-Shabazz, Malcolm LittleBorn: May 19, 1925 in Omaha, NebraskaParents: Rev. Earl Little, Louise LittleDied: February 21, 1965 in New York, New YorkEducation: Through grade eightPublished Works: The Autobiography of Malcolm  XAwards and Honors:  Multiple historic markers and plaques; streets and schools named in his honor; stamp produced with his likenessSpouse: Betty SandersChildren: Attallah, Qubilah, Ilyasah, Gamilah, Malikah, MalaaknNotable Quote: â€Å"The white man is afraid of truth†¦ I’m the only black man they’ve ever been close to who they know speaks the truth to them. Its their guilt that upsets them, not me.† Early Life of Malcolm X Malcolm X was born as Malcolm Little in Omaha, Nebraska to Earl and Louise Little (neà © Norton). Earl was a Baptist minister and also worked for Marcus Garveys Universal Negro Improvement Association (UNIA), a pan-African movement in the 1920s. Louise, who had grown up in Grenada, was Earls second wife. Malcolm was the fourth of the six children Louise and Earl shared. (Earl also had three children from his first marriage.) As a kid, Malcolm would often attend UNIA meetings with his father, who was president of the Omaha chapter at one point, absorbing Garveys argument that the African-American community had the tools and resources to blossom without dependence on the white man. Earl Little challenged the social standards of the time. When he began to attract the attention of the Ku Klux Klan, he moved his family to a white neighborhood in Lansing, Michigan. Neighbors protested. On November 8, 1929, a group of white supremacists known as the Black Legion set fire to the Littles home with Malcolm and his family inside. Luckily, the Littles managed to escape but then watched their house burn to the ground while firemen did nothing to put out the flames. Despite the seriousness of the threats against him, Earl did not let intimidation silence his beliefs—and this almost certainly cost him his life. Malcolm X’s Father Is Murdered While the details of his death remain uncertain, what is known is that Earl was murdered on September 28, 1931 (Malcolm was only 6 years old). Earl had been savagely beaten and then left on trolley tracks, where he was run over by a trolley. Although those responsible were never found, the Littles always believed the Black Legion was responsible. Realizing he was likely to meet a violent end, Earl had purchased life insurance; however, the life insurance company ruled his death a suicide and refused to pay. These events plunged Malcolms family into poverty. Louise tried to work, but this was during the Great Depression and there weren’t many jobs for the widow of a black activist. Welfare was available, but Louise didn’t want to take charity. Things were tough in the Little home. There were six children and very little money or food. The strain of taking care of everyone by herself started to take its toll on Louise and by 1937, she was showing signs of becoming mentally ill. In January 1939, Louise was committed to the State Mental Hospital in Kalamazoo, Michigan. Malcolm and his siblings were divided up. Malcolm was one of the first to go, even before his mother was institutionalized. In October 1938, 13-year-old Malcolm was sent to a foster home, which was soon followed by a detention home. Despite his unstable home life, Malcolm was a success at school. Unlike the other kids at the detention home who were sent to a reform school, Malcolm was allowed to attend Mason Junior High School, the only regular junior high in town. While at junior high, Malcolm earned top grades even against his white classmates. However, when a white teacher told Malcolm that he couldn’t become a lawyer but should instead consider becoming a carpenter, Malcolm was so disturbed by the comment that he began withdrawing from those around him. When Malcolm met his half-sister Ella for the first time, he was ready for a change. Drugs and Crime Ella was a confident, successful young woman living in Boston at the time. When Malcolm asked to go live with her, she agreed. In 1941, having just finished the eighth grade, Malcolm moved from Lansing to Boston. While exploring the city, he befriended a hustler named â€Å"Shorty† Jarvis, who also happened to come from Lansing. Shorty got Malcolm a job shining shoes at the Roseland Ballroom, where top bands of the day played. Malcolm soon learned that his customers also hoped he could supply them with marijuana. It wasn’t long before Malcolm was selling drugs as well as shining shoes. He also personally started to smoke cigarettes, drink liquor, gamble, and do drugs. Dressing in zoot suits and â€Å"conking† (straightening) his hair, Malcolm loved the fast life. He then moved to Harlem in New York and began getting involved in petty crimes and selling drugs. Soon, Malcolm himself developed a drug habit (cocaine) and his criminal behavior escalated. After several run-ins with the law, Malcolm was arrested in February 1946 for burglary and sentenced to 10 years in prison. He was sent to the Charlestown State Prison in Boston. Prison Time and the Nation of Islam In late 1948, Malcolm was transferred to the Norfolk, Massachusetts Prison Colony. It was there that Malcolms brother Reginald introduced him to the Nation of Islam (NOI). Originally founded in 1930 by Wallace D. Fard, the Nation of Islam was a black Muslim organization that believed blacks were inherently superior to whites and predicted the destruction of the white race. After Fard mysteriously disappeared in 1934, Elijah Muhammad took over the organization, calling himself the â€Å"Messenger of Allah.† Malcolm believed in what his brother Reginald told him. Through personal visits and many letters from Malcolm’s siblings, Malcolm began to learn more about the NOI. Using Norfolk Prison Colony’s extensive library, Malcolm rediscovered education and began reading extensively. With his ever increasing knowledge, Malcolm began writing to Elijah Muhammad daily. By 1949, Malcolm had converted to the NOI, which required purity of body—eliminating Malcolms drug habit. In 1952, Malcolm emerged from prison a devoted follower of the NOI and a proficient writer, two essential factors in changing his life. Becoming an Activist Once out of prison, Malcolm moved to Detroit and began recruiting for the NOI. Elijah Muhammad, the leader of the NOI, became Malcolms mentor and hero, filling the void Earls death had left. In 1953, Malcolm adopted the NOI’s tradition of replacing one’s last name (which was thought to have been forced upon an ancestor by their white slave-owner) with the letter X, a reference to the unknown heritage complicating African-American identity. Charismatic and passionate, Malcolm X rose quickly in the NOI, becoming the minister of the groups Temple Seven in Harlem in June 1954. Malcolm X simultaneously was becoming an accomplished journalist; he wrote for several publications before he founded the NOIs newspaper, Muhammad Speaks. While working as the minister of Temple Seven, Malcolm X noticed that a young nurse named Betty Sanders had started attending his lectures. Without ever having gone on an individual date, Malcolm and Betty got married on January 14, 1958. The couple went on to have six daughters; the last two were twins who were born after Malcolm X’s assassination. America Encounters Malcolm X Malcolm X soon became a visible figure in the NOI, but it was the wonder of television that brought him national attention. When CBS aired the documentary Nation of Islam: The Hate That Hate Produced, in July of 1959, Malcolm Xs dynamic speech and obvious charm reached a national audience. Malcolm Xs radical claims of black superiority and refusal to accept non-violent strategies got him interviews across the social spectrum. Malcolm X had become a national figure and the de facto face of the NOI. While Malcolm X became well-known, he was not necessarily liked. His views unsettled much of America. Many in the white community feared that Malcolm Xs doctrine would incite mass violence against whites. Many in the black community were concerned that Malcolm X’s militancy would destroy the growing effectiveness of the non-violent, mainstream Civil Rights Movement. Malcolm X’s newfound fame also attracted the attention of the FBI, which soon began tapping his phone over concerns that some kind of racially based revolution was brewing. Malcolm Xs meetings with Cuban Communist leader Fidel Castro did little to alleviate these fears. Trouble Within the NOI By 1961, Malcolm Xs meteoric rise within the organization as well as his new celebrity status had become a problem within the NOI. Simply stated, other ministers and members of the NOI had become jealous. Many began insinuating that Malcolm X was financially profiting from his position and that he intended to take over the NOI from Muhammad. This jealousy and envy bothered Malcolm X, but he tried to put it out of his mind. In 1962, rumors about improprieties by Muhammad began to reach Malcolm X. To Malcolm X, Muhammad was not only a spiritual leader but also a moral example for all to follow. It was this moral example that had helped Malcolm X escape his drug addiction and keep him abstinent for 12 years (from the time of his prison sentence to his marriage). Thus, when it became obvious that Muhammad had engaged in immoral behavior, including fathering four illegitimate children, Malcolm X was devastated by his mentors deception. Things Get Worse After President John F. Kennedy was assassinated on November 22, 1963, Malcolm X, never one to shy away from conflict, publicly interpreted the event as the chickens coming home to roost. Malcolm X claimed that he meant the feelings of hate within America were so great that they had spilled over from the conflict between black and white and ended up causing the killing of the president. However, his comments were interpreted as support for the death of the  beloved Kennedy, a Democrat from Massachusetts. Muhammad, who had specifically ordered all his ministers to remain silent regarding Kennedy’s assassination, was very unhappy over the negative publicity. As punishment, Muhammad ordered Malcolm X to be â€Å"silenced† for 90 days. Malcolm X accepted this punishment, but he soon discovered that Muhammad intended to push him out of the NOI. In March 1964, the internal and external pressure became too much and Malcolm X announced that he was leaving the Nation of Islam, an organization he had worked so hard to grow. Returning to Islam After leaving the NOI in 1964, Malcolm decided to found his own religious organization, Muslim Mosque, Inc. (MMI), which catered to former NOI members. Malcolm X turned to traditional Islam to inform his path. In April 1964, he began a pilgrimage (or hajj) to Mecca in Saudi Arabia. While in the Middle East, Malcolm X was amazed by the diversity of complexions represented there. Even before returning home, he began to rethink his earlier divisive positions and decided to prioritize faith over skin color. Malcolm X symbolized this shift by changing his name once again, becoming El-Hajj Malik El-Shabazz. Malcolm X then toured Africa, where the early influence of Marcus Garvey reemerged. In May 1964, Malcolm X began his own pan-African movement with the Organization of Afro-American Unity (OAAU), a secular organization that advocated for human rights for all those of African descent. As head of the OAAU, Malcolm X met with world leaders to forward this mission, generating a far more diverse following than the NOI. Whereas once he had shunned all of white society, he now encouraged interested whites to teach about oppression. Running both the MMI and the OAAU exhausted Malcolm, but both spoke to passions that defined him—faith and advocacy. Death Malcolm Xs philosophies had changed dramatically, bringing him more in line with the mainstream Civil Rights movement. However, he still had enemies. Many in the NOI felt he had betrayed the movement when he publicly discussed Muhammads adultery. On February 14, 1965, Malcolm Xs New York home was firebombed. He believed the NOI was responsible. Still ever defiant, Malcolm X did not let this attack interrupt his schedule. He traveled to Selma, Alabama and returned to New York for a speaking engagement at the Audubon Ballroom in Harlem on February 21, 1965. This was Malcolm Xs last speech. Once Malcolm was at the podium, a commotion in the middle of the crowd drew attention. While everyone was focused on the commotion, Talmadge Hayer and two other NOI members stood up and shot Malcolm X. Fifteen bullets hit their target, killing Malcolm X. He was dead before he reached the hospital. The chaos that broke out at the scene spilled into the streets of Harlem as mob violence and the firebombing of a Black Muslim mosque followed. Malcolms critics, including Elijah Muhammad, maintained that he died by the very violence he defended in his early career. Talmadge Hayer was arrested at the scene and two other men were taken into custody shortly after. All three would be convicted of the murder; however, many believe the other two men were not guilty. Many questions remain about the assassination; specifically, who really carried out the shooting and who ordered the assassination in the first place? Legacy In the month prior to his death, Malcolm X had been dictating his biography to noted African-American author Alex Haley. The Autobiography of Malcolm X was published in 1965, just months after Malcolm Xs murder. Through his autobiography, Malcolm X’s powerful voice continued to inspire the black community to advocate for their rights. The Black Panthers, for example, used Malcolm X’s teachings to found their own organization in 1966. Today, Malcolm X remains one of the more controversial figures of the Civil Rights era. He is generally respected for his passionate demand for change in one of historys most trying (and deadly) times for black leaders. Sources The Autobiography of Malcolm  X. With the assistance of Alex Haley. New York: Grove Press, 1965. Mamiya, Lawrence. XMalcom. Encyclopà ¦dia Britannica, 1 February 2019. Remnick, David. â€Å"This American Life: The making and remaking of Malcolm X.† The New Yorker, The New Yorker, 19 June 2017.